Free Content Spectral "fingerprinting" of phytoplankton populations by two-dimensional fluorescence and Fourier-transform-based pattern recognition

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Abstract:

The selectivity of fluorescence spectroscopy is exploited for the characterization of marine algae. Two-dimensional, digital images of in vivo fluorescence intensity versus excitation and emission wavelengths, called excitation-emission matrices (EEMs), are used as spectral "fingerprints" for marine phytoplankton populations. Fourier-transform-based pattern recognition is described along with its inherent strengths and weaknesses for the analysis of natural populations. The EEMs of unknown algae are compared to a library of standard EEMs representing 23 algal species and 6 classes with better than 80% accuracy. The EEMs acquired under different physiological conditions are used in determining pattern recognition reliability. The potential for fingerprinting mixed populations and oceanographic regions is also discussed.

Document Type: Research Article

DOI: http://dx.doi.org/10.1357/002224085788453903

Publication date: November 1, 1985

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  • The Journal of Marine Research publishes peer-reviewed research articles covering a broad array of topics in physical, biological and chemical oceanography. Articles that deal with processes, as well as those that report significant observations, are welcome. In the area of biology, studies involving coupling between ecological and physical processes are preferred over those that report systematics. Authors benefit from thorough reviews of their manuscripts, where an attempt is made to maximize clarity. The time between submission and publication is kept to a minimum; there is no page charge.
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