Domestication of microbially enhanced coalbed methane microorganism
A methanogenic strain was obtained from anaerobic sludge.The strain was cultivated anaerobically with coal as solo carbon resource.The methanogenesis regulation of coal consumed by the strain and the influence of conventional carbonaceous substrates on gas production were investigated.It
is found that adaptive phase is decreased from 15 days of original strain to 6 days of acclimated strain.The gas production is greatly raised as well.A 28 days’ gas production cycle can be divided into three phasesadaptive phasegasproducing phase and stable phase.The total amount of
gas produced by 100 mL substrate is 182 mL which comprises 16% methane.Daily gas production increases at the beginning and then decreases.During 15th day to 17th daygas production reachs the peak amount of 20 mL/day.The strain can also make use of sodium acetate and methanol to produce gas.Howeversodium
acetate has a better prompting effect on gas production of coal consumed by the strain.
Keywords: coalbed methane; increase production; methanogen; microorganism domestication
Document Type: Research Article
Publication date: 15 September 2012
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