Lipoarabinomannan enzyme-linked immunosorbent assay for early diagnosis of childhood tuberculous meningitis
OBJECTIVE: To evaluate the diagnostic value of a commercial, antigen-capture enzyme-linked immunosorbent assay (ELISA) test based on the detection of LAM in urine for the early diagnosis of TBM in children.
METHOD: A cross-sectional study in which urine samples from paediatric patients with suspected TBM attending the Tygerberg Children's Hospital, Cape Town, South Africa, were tested for LAM.
RESULTS: Complete data were available for 50 of 56 patients with suspected TBM. TBM was diagnosed in 21 (42%) patients and excluded in 29 (58%). The LAM ELISA had a sensitivity of 4.8% and a specificity of 93.1%. Serial measurements in the first 2 weeks after treatment initiation did not improve test performance.
CONCLUSION: We have shown that urinary LAM detection was of little value for the diagnosis of TBM in a cohort of paediatric patients with suspected TBM.
Document Type: Research Article
Affiliations: 1: Department of Paediatric Infectious Diseases and Immunology, VU University Medical Center, Amsterdam, The Netherlands 2: Department of Paediatrics and Child Health, Stellenbosch University, Cape Town, South Africa 3: Department of Paediatric Infectious Diseases and Immunology, VU University Medical Center, Amsterdam, The Netherlands; Department of Paediatrics and Child Health, Stellenbosch University, Cape Town, South Africa 4: KIT Biochemical Research, Royal Tropical Institute, Amsterdam, The Netherlands
Publication date: 2014-02-01
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