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Capilia test for identification of Mycobacterium tuberculosis in MGIT™-positive cultures

Authors: Gomathi, N. S.1; Devi, S. M.1; Lakshmi, R.1; Ramachandran, R.2; Wares, D. F.2; Kumar, V.1; Selvakumar, N.1

Source: The International Journal of Tuberculosis and Lung Disease, Volume 16, Number 6, 1 June 2012 , pp. 788-792(5)

Publisher: International Union Against Tuberculosis and Lung Disease

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Abstract:

BACKGROUND: The performance of the Capilia test for rapid identification of Mycobacterium tuberculosis complex (MTC) in Mycobacterium Growth Indicator Tube (MGIT) positive samples with contaminating organisms is not well documented.

OBJECTIVE: To assess the diagnostic yield of the Capilia test in the rapid identification of MTC in MGIT-positive cultures.

DESIGN: A total of 459 selected sputum samples were cultured using BACTEC™ MGIT™ 960. Tubes flagged positive by the MGIT instrument (MGIT-positive) were examined for acid-fast bacilli and cording in smears, spotted on blood agar (BA), subcultured for biochemical tests and tested using the Capilia test. Based on smear and growth on BA, MGIT-positive tubes were grouped into MGIT true-positive, MGIT-positive with contamination and MGIT contamination. Performance parameters of Capilia test such as sensitivity, specificity, efficiency, and positive and negative predictive values (PPV, NPV) for each of these groups were determined against biochemical tests as gold standard.

RESULTS: Of the 346 MGIT-positives, respectively 233, 73 and 40 were MGIT true-positive, MGIT-positive with contamination and MGIT contamination. For the three groups, the PPV and NPV of the Capilia test were respectively 97%, 96% and 100%, and 32%, 27% and 60%.

CONCLUSION: In settings with high contamination of MGIT cultures, the performance of the Capilia test is diminished.

Keywords: Capilia; M. tuberculosis; MGIT™ 960; rapid identification

Document Type: Research Article

DOI: http://dx.doi.org/10.5588/ijtld.11.0356

Affiliations: 1: National Institute for Research in Tuberculosis, Chennai, India 2: Office of the World Health Organization Representative to India, New Delhi, India

Publication date: June 1, 2012

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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