Real-time PCR and Amplified MTD® for rapid detection of Mycobacterium tuberculosis in pulmonary specimens
OBJECTIVE: To evaluate the ‘Care TB®’ real-time polymerase chain reaction (PCR) assay in comparison with the Amplified MTD® Test (AMTD) for the rapid diagnosis of TB.
DESIGN: Self-expectorated sputum was collected for direct smear microscopy, culture, real-time PCR and AMTD assay. Performance of the ‘Care TB’ real-time PCR and AMTD assay were compared using a combination of culture and clinical diagnosis as a reference standard.
RESULTS: Of the 178 sputum specimens, 83 were culture-positive; of these, 74 were Mycobacterium tuberculosis complex and 9 strains were non-tuberculous mycobacteria. The overall sensitivities and specificities were respectively 91.6% and 100% for real-time PCR, and 95.2% and 97.9% for AMTD. In the smear-positive specimens, the sensitivities and specificities were respectively 97% and 100% for real-time PCR, and 98.5% and 100% for AMTD. In the smear-negative specimens, the sensitivities and specificities were 70.6% and 100% for real-time PCR, and 82.4% and 97.7% for AMTD.
CONCLUSIONS: Both real-time PCR and AMTD are rapid and specific tests for detecting M. tuberculosis complex; however, ‘Care TB’ real-time PCR is more convenient and economical.
Document Type: Research Article
Affiliations: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China
Publication date: 2012-02-01
The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.
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