Skip to main content

Free Content Real-time PCR and Amplified MTD® for rapid detection of Mycobacterium tuberculosis in pulmonary specimens

Download Article:
 Download
(PDF 272.6083984375 kb)
 
SETTING: Patients with suspected pulmonary tuberculosis (PTB) in the First Affiliated Hospital, College of Medicine, Zhejiang University.

OBJECTIVE: To evaluate the ‘Care TB®’ real-time polymerase chain reaction (PCR) assay in comparison with the Amplified MTD® Test (AMTD) for the rapid diagnosis of TB.

DESIGN: Self-expectorated sputum was collected for direct smear microscopy, culture, real-time PCR and AMTD assay. Performance of the ‘Care TB’ real-time PCR and AMTD assay were compared using a combination of culture and clinical diagnosis as a reference standard.

RESULTS: Of the 178 sputum specimens, 83 were culture-positive; of these, 74 were Mycobacterium tuberculosis complex and 9 strains were non-tuberculous mycobacteria. The overall sensitivities and specificities were respectively 91.6% and 100% for real-time PCR, and 95.2% and 97.9% for AMTD. In the smear-positive specimens, the sensitivities and specificities were respectively 97% and 100% for real-time PCR, and 98.5% and 100% for AMTD. In the smear-negative specimens, the sensitivities and specificities were 70.6% and 100% for real-time PCR, and 82.4% and 97.7% for AMTD.

CONCLUSIONS: Both real-time PCR and AMTD are rapid and specific tests for detecting M. tuberculosis complex; however, ‘Care TB’ real-time PCR is more convenient and economical.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: AMTD; real-time PCR; tuberculosis

Document Type: Research Article

Affiliations: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China

Publication date: 2012-02-01

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more