Genetic polymorphisms in the P2X7 gene and its association with susceptibility to tuberculosis
OBJECTIVE: To assess the role of P2X7 gene −762T/C and −1513A/C polymorphisms in TB susceptibility.
DESIGN: In a case-control study, polymerase chain reaction (PCR) amplification of genomic DNA extracted from peripheral blood samples of cases and controls was followed by restriction fragment length polymorphism or allele-specific PCR.
RESULTS: The 1513C allele was found to be associated with TB susceptibility (P = 0.02, OR for variant C allele 1.33, 95%CI 1.03–1.73). A significant protective association against TB was found for −762T/C polymorphism (P < 0.01, OR for variant C allele 0.72, 95%CI 0.57–0.91). On classifying samples on the basis of sex, only males showed a significant association between P2X7 −1513A/C and P2X7 −762T/C and TB in the present study.
CONCLUSION: A significant association of 762T/C and 1513A/C polymorphisms with TB in the P2X7 gene was found in our study population. A sex bias, with only males showing a significant association with the disease, is the first report of this kind. Genetic studies for the characterisation of the susceptibility genes can help to gain a better understanding of TB pathogenesis.
Document Type: Research Article
Affiliations: 1: Department of Biotechnology, Punjab University, Chandigarh, India 2: Department of Pulmonary Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India 3: Department of Biotechnology, Shri Guru Gobind Singh College, Chandigarh, India 4: Department of Biotechnology, Goswami Ganesh Dutta Sanatan Dharma College, Chandigarh, India
Publication date: 2012-02-01
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