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Free Content Rapid molecular identification of mycobacterial species in positive culture isolates using the biochip test

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SETTING: Patients infected with non-tuberculous mycobacteria usually fail treatment due to erroneous diagnoses. Early detection of mycobacterial species is essential for adequate case management.

OBJECTIVE: To conduct a multicentre, prospective evaluation of the recently developed biochip test and to determine its sensitivity and specificity in three clinical hospitals in China.

RESULTS: A total of 1565 clinical isolates obtained from three hospitals were identified as 19 mycobacterial species by 16S sequencing. The same 53 reference strains were detected in all three hospitals as quality control and to evaluate the reproducibility of the assay. The overall accuracy of the species identification assay among all strains was 99.9% (1722/1724). The two unidentified samples, belonging to Mycobacterium parascrofulaceum and M. monacense, were not included among the 17 mycobacterial species. The reference strains demonstrated that the reproducibility of the assay was 100%.

CONCLUSION: The biochip test provided cost-effective and highly sensitive identification of mycobacterial species in less than 6 h. This will help clinical staff carry out more efficient and personalised clinical treatment without delay.
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Keywords: BIOCHIP; MYCOBACTERIA; NTM

Document Type: Research Article

Affiliations: 1: National Center for Tuberculosis Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China 2: Institute of Pulmonary Diseases of Guangzhou Chest Hospital, Guangzhou, China 3: Key Laboratory of Mycobacteria Tuberculosis, Shanghai Pulmonary Hospital, Tongji University, Shanghai, China 4: TB Reference Laboratory, Department of Health, Hong Kong Special Administrative Region, China

Publication date: 01 December 2011

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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