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Free Content Genotyping and drug resistance patterns of Mycobacterium tuberculosis strains in five provinces of China

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OBJECTIVE: To evaluate the distribution of the Mycobacterium tuberculosis Beijing genotype and the association of the genotype with drug-resistant M. tuberculosis strains in five provinces in China.

DESIGN: M. tuberculosis strains (n = 158) isolated from five provinces of China were subjected to insertion sequence 6110 restriction fragment length polymorphism (RFLP), spoligotyping and mycobacterial interspersed repetitive units (MIRU) analyses. The prevalence of the Beijing genotype strains in each province was determined and compared. The proportion method was used to test the drug susceptibility of all strains.

RESULT: Of the 158 strains, 123 (77.8%) were identified as the Beijing genotype by RFLP and spoligotyping. Nearly all the strains (n = 152, 96.2%) were grouped into 14 shared spoligotypes. Six other spoligotypes were unique to China. The prevalence of the Beijing genotype was significantly higher in the interior than in coastal areas (P < 0.001, OR 5.4, 95%CI 2.3–12.7). Resistance to rifampicin (RMP) was associated with the Beijing strain (P = 0.05, OR 3.7, 95%CI 1.2–11.1).

CONCLUSION: The M. tuberculosis Beijing genotype varies in prevalence in different regions of China and is solely associated with RMP resistance.

Keywords: DNA fingerprinting; Mycobacterium tuberculosis; drug resistance

Document Type: Regular Paper


Affiliations: 1: Beijing Tuberculosis and Thoracic Tumour Research Institute, Beijing, China 2: Beijing Research Institute for Tuberculosis Control, Beijing, China

Publication date: 2011-06-01

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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