Molecular genotyping of Mycobacterium tuberculosis in Xi'an, China, using MIRU-VNTR typing
OBJECTIVE: To characterize the population of M. tuberculosis strains circulating in Xi'an and to evaluate the feasibility of the mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) typing method.
DESIGN: A total of 195 M. tuberculosis clinical isolates from Xi'an were genotyped using spoligotyping and MIRU-VNTR.
RESULTS: Thirty-two different genotypes were identified by spoligotyping, with the Beijing family identified as the largest lineage (84.6%), followed by the T1 (3.08%), T2 (1.54%), H3 (1.03%) and U (1.03%) families. The Hunter-Gaston discriminatory index (HGDI) of spoligotyping is low (HGDI = 0.392). In contrast, 15-locus MIRU-VNTR shows a higher discriminatory power (HGDI = 0.998) than 12-locus MIRU-VNTR (HGDI = 0.948). By replacing the ETR C locus with the MIRU39 locus, the discriminatory power of 15-locus MIRU-VNTR is increased to 0.999.
CONCLUSIONS: The Beijing family accounts for the vast majority of M. tuberculosis isolates in Xi'an, China. The modified 15-locus MIRU-VNTR showed high HGDI and can be used as a first-line genotyping method in combination with spoligotyping in routine epidemiological investigations in Xi'an, China.
Document Type: Regular Paper
Affiliations: 1: Department of Immunology and Pathogenic Biology, School of Medicine, Xi'an Jiao Tong University, Xi'an, China; and Department of Medical Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai, China 2: Department of Immunology and Pathogenic Biology, School of Medicine, Xi'an Jiao Tong University, Xi'an, China 3: Xi'an Chest and Tuberculosis Hospital, Xi'an, China 4: Center for Tuberculosis Research, Division of Infectious Diseases, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 5: Department of Medical Microbiology and Parasitology, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Publication date: 2011-04-01
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