If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Free Content Diagnosis of latent tuberculosis infection in bacille Calmette-Guérin vaccinated subjects in China by interferon-gamma ELISpot assay

 Download
(PDF 439.8kb)
 
Download Article:

Abstract:

OBJECTIVE: To evaluate the performance of an in-house interferon-gamma (IFN-γ) enzyme-linked immunospot (ELISpot) assay for the detection of latent tuberculosis infection (LTBI) in bacille Calmette-Guérin (BCG) vaccinated individuals with or without human immunodeficiency virus (HIV) infection.

METHODS: A total of 202 participants (93 HIV-infected and 109 non-infected) who received BCG vaccination at birth underwent tuberculin skin testing (TST) and T cell-based ELISpot assays. The performance of an in-house IFN-γ ELISpot assay (ELISpot) was evaluated by parallel comparison with the commercial IFN-γ release assay (IGRA) kit, T-SPOT®.TB, in 127 subjects. The effect of BCG vaccination on the ELISpot assay was prospectively determined by comparing the responses of IGRAs before and 3 months after BCG vaccination in 27 individuals.

RESULTS: High agreement between ELISpot and T-SPOT.TB was observed in both HIV-infected (92.47%, κ = 0.754) and non-HIV-infected subjects (97.06%, κ = 0.653). CD4+ T-cell count does not affect Mycobacterium tuberculosis IFN-γ response as measured by both ELISpot and T-SPOT.TB. BCG vaccination resulted in 100% conversion of TST, but did not change responses to IGRAs.

CONCLUSION: The performance of this in-house ELISpot assay is comparable to commercial T-SPOT.TB in diagnosing LTBI. Both assays are useful for diagnosing LTBI in BCG-vaccinated subjects with or without HIV infection in a setting with a high prevalence of tuberculosis.

Keywords: BCG vaccination; interferon-gamma release assays; latent tuberculosis infection

Document Type: Regular Paper

Affiliations: 1: Shenzhen Clinical Centre for Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen, China 2: Department of Immunology, Affiliated Shenzhen Third Hospital, Guangdong Medical College, Shenzhen, China 3: Shenzhen Polytechnic College, Shenzhen, China 4: Department of Neurosurgery, University of Colorado Denver, Aurora, Colorado, USA 5: Shenzhen Clinical Centre for Infectious Diseases, Shenzhen Third People's Hospital, Shenzhen, China; and Department of Immunology, Affiliated Shenzhen Third Hospital, Guangdong Medical College, Shenzhen, China

Publication date: December 1, 2010

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more