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Rapid detection of immunoglobulin G against Mycobacterium tuberculosis antigens by two commercial ELISA kits

Authors: Ben Selma, W.1; Harizi, H.1; Marzouk, M.1; Ben Kahla, I.1; Ben Lazreg, F.1; Ferjeni, A.1; Harrabi, I.2; Abdelghani, A.3; Ben Said, M.1; Boukadida, J.1

Source: The International Journal of Tuberculosis and Lung Disease, Volume 14, Number 7, July 2010 , pp. 841-846(6)

Publisher: International Union Against Tuberculosis and Lung Disease

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SETTING: Tunisia.

OBJECTIVE: To assess the clinical usefulness of the commercial Pathozyme-Myco G® (Myco G) and Pathozyme TB complex plus® (Patho) enzyme-linked immunosorbent assay (ELISA) kits for the rapid diagnosis of active tuberculosis (TB) and to distinguish between active TB and non-TB pulmonary diseases in Tunisian patients.

DESIGN: Immunoglobulin G mediated humoral immune response against mycobacterial antigens (38 kDa and lipoarabinomannan, Myco G; 16 and 38 kDa, Patho) was evaluated in a group of active TB patients (128 smear-positive pulmonary and 33 extra-pulmonary samples) and in a control group (107 patients with non-tuberculous lung disease and two with leprosy). Active TB cases were confirmed by Mycobacterium tuberculosis culture from clinical samples.

RESULTS: The sensitivity of the Myco G test was 71% in active TB (pulmonary and extra-pulmonary), while the specificity was 100%. The Patho test showed a sensitivity of 43.5% with a specificity of 96.3%. A combination of both tests showed a sensitivity of 81% and a specificity of 96.3%.

CONCLUSIONS: Both ELISA tests were simple and easy to perform. Their combined use led to an increase in the diagnostic accuracy of active TB and its discrimination from non-TB pulmonary diseases. They could therefore be used as screening tools in poorly equipped laboratories in TB-endemic regions.

Keywords: Mycobacterium tuberculosis; diagnosis; mycobacterial antigens; tuberculosis

Document Type: Regular Paper

Affiliations: 1: Microbiology and Immunology Laboratory, UR02SP13, Centre Hospitalier Universitaire Farhat Hached, Sousse, Tunisia 2: Epidemiology Unit, Centre Hospitalier Universitaire Farhat Hached, Sousse, Tunisia 3: Pneumology Unit, Centre Hospitalier Universitaire Farhat Hached, Sousse, Tunisia

Publication date: July 1, 2010

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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