Skip to main content

Free Content Effector memory T-cells dominate immune responses in tuberculosis treatment: antigen or bacteria persistence?

Download Article:
(PDF 830.798828125 kb)
OBJECTIVE: To compare ex vivo immunological responses upon stimulation of lymphocytes with Mycobacterium tuberculosis-specific antigens in three groups: 1) subjects diagnosed with tuberculosis (TB) in the early 1940s and 1950s but who did not receive anti-tuberculosis chemotherapy (n = 5), 2) subjects treated with anti-tuberculosis agents prior to the rifampicin (RMP) era (n = 26) and 3) subjects who received RMP as a part of modern combination therapy (n = 7).

DESIGN: A total of 38 healthy subjects, mean age 70 ± 13 years, with a history of previously treated TB were recruited. Peripheral blood mononuclear cells were collected and analysed as a batch by ELISpot. Representative samples with high reactivities were further immunophenotypically characterised.

RESULTS: No differences between the studied groups were detected with regard to the frequencies of reactive lymphocytes. The dominant immunophenotypic profile of the representative responders, irrespective of the treatment schemes, was CD4+CD45RO+CD45RA−CD27−CD28−CCR7−, compatible with the fast reacting effector memory T-cell lineage (TEM).

CONCLUSION: Specific TEM cells persist even in subjects treated for TB decades ago with modern anti-tuberculosis chemotherapy. Additional studies are needed to address the question of what drives the survival of TEM after adequate treatment: persistence of antigens or bacteria.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Mycobacterium tuberculosis; effector T-cells; immunophenotyping; memory T-cells; tuberculosis

Document Type: Regular Paper

Affiliations: 1: Haartman Institute, Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland 2: Länsi-Uusimaa Hospital, Tammisaari, Finland 3: Haartman Institute, Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland; and Division of Clinical Microbiology, HUSLAB Laboratory Services, Helsinki University Hospital, Helsinki, Finland

Publication date: 2010-03-01

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more