Authors: Kourout, M.¹; Chaoui, I.²; Sabouni, R.³; Lahlou, O.³; El Mzibri, M.²; Jordaan, A.⁴; Victor, T.C.⁴; Akrim, M.¹; El Aouad, R.⁵

Source: The International Journal of Tuberculosis and Lung Disease, Volume 13, Number 11, November 2009 , pp. 1440-1442(3)

Publisher: International Union Against Tuberculosis and Lung Disease

Abstract:

Mutations in the rpoB gene associated with rifampicin (RMP) resistance were studied in 47 RMP-resistant and 147 RMP-susceptible clinical strains of Mycobacterium tuberculosis from Morocco using probe-based assay and DNA sequencing. RMP-resistant mutations were identified in 85% of RMP-resistant isolates. No mutations were observed among the 147 RMP-susceptible strains. Sequence analysis identified 10 alleles, including two deletions not previously reported. Nucleotide changes at codons 531, 526 and 516 were the most prominent, accounting for 74.4% of our RMP-resistant strains. These results demonstrate that resistance genotyping at these codons would be a good marker for the rapid detection of RMP resistance.

Keywords: Mycobacterium tuberculosis; rpoB gene; rifampicin resistance marker; Morocco

Document Type: Short communication

Affiliations: 1: Laboratoire de Biologie Moléculaire, Institut National d'Hygiène, Rabat, Morocco 2: Unité de Biologie et Recherche Médicales, Centre National de l'Energie des Sciences et Techniques Nucléaires, Rabat, Morocco 3: Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat, Morocco 4: Department of Medical Biochemistry, Stellenbosch University, Tygerberg, South Africa 5: Laboratoire de Biologie Moléculaire, Institut National d'Hygiène, Rabat, Morocco; and Laboratoire de Référence de la Tuberculose, Institut National d'Hygiène, Rabat, Morocco

Publication date: 2009-11-01

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• The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

  Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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