Skip to main content

Free Content Adenosine deaminase activity and its isoenzymes in the sputum of patients with pulmonary tuberculosis

Download Article:
(PDF 269.166015625 kb)
BACKGROUND: Adenosine deaminase (ADA) has been widely used for the diagnosis of tuberculous pleural effusion. Two isoenzymes have been described, ADA1 and ADA2.

OBJECTIVE: To evaluate the diagnostic value of sputum ADA, ADA1 and ADA2 activity in pulmonary tuberculosis (TB).

DESIGN: We measured total ADA, ADA1 and ADA2 activity in the sputum of 27 patients with pulmonary TB (11 had a negative Ziehl-Neelsen stain for acid-fast bacilli [AFB]). Nineteen patients with lung cancer were used as controls.

RESULTS: Sputum total ADA activity was significantly higher in TB than in lung cancer patients (median 18 U/l [range 3–70] vs. 6 U/l [2–16]; P < 0.001). Sputum ADA2 activity was significantly higher in TB compared to lung cancer patients (9 U/l [0–65] vs. 5 U/l [0–12]; P = 0.001). Sputum ADA2 was significantly higher than ADA1 in TB patients (P = 0.001). Sputum ADA and ADA2 were higher in both AFB-positive and AFB-negative TB patients. Using a cut-off level of respectively 16 UI/l and 5UI/l for sputum total ADA and ADA2, sensitivity and specificity were 55.6% and 100% for total ADA and 81.5% and 63.2% for ADA2.

CONCLUSION: Sputum total ADA and ADA2 levels are elevated in patients with pulmonary TB. As they are elevated even in AFB-negative patients, they may assist in the early diagnosis of pulmonary TB.
No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: ADA; sputum; tuberculosis

Document Type: Regular Paper

Affiliations: 1: Sixth Department of Respiratory Medicine, Sotiria Chest Hospital, Athens, Greece 2: Department of Respiratory and Critical Care Medicine, Sotiria Chest Hospital, Athens, Greece

Publication date: 2009-06-01

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more