Authors: Affolabi, D.¹; Anyo, G.²; Faïhun, F.³; Sanoussi, N.³; Shamputa, I.C.²; Rigouts, L.²; Kestens, L.⁴; Anagonou, S.³; Portaels, F.²

Source: The International Journal of Tuberculosis and Lung Disease, Volume 13, Number 3, March 2009 , pp. 317-322(6)

Publisher: International Union Against Tuberculosis and Lung Disease

Abstract:

OBJECTIVES: To assess the diversity of Mycobacterium tuberculosis strains in Cotonou, Benin, and the risk factors associated with clustering.

METHODS: We analysed one sputum sample from 194 consecutive new pulmonary tuberculosis (TB) cases using two genotyping methods: spoligotyping and the 12 loci mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTR). The data obtained were compared to the SpolDB4.0 database.

RESULTS: We have found that spoligotype 61, highly predominant in West Africa, was also the most prevalent strain in Cotonou. We observed that the Beijing family represented 10.3% of strains and was associated with resistance to streptomycin. We also confirmed that combining spoligotyping and MIRU-VNTR provided a higher discriminatory power than the two techniques used individually.

CONCLUSION: Spoligotype 61 and Beijing genotype are the most prevalent genotypes of M. tuberculosis in Cotonou.

Keywords: molecular epidemiology; Mycobacterium tuberculosis; Cotonou

Document Type: Regular paper

Affiliations: 1: Laboratoire de Référence des Mycobactéries, Cotonou, Bénin; Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium 2: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium 3: Laboratoire de Référence des Mycobactéries, Cotonou, Bénin 4: Immunology Unit, Institute of Tropical Medicine, Antwerp, Belgium

Publication date: 2009-03-01

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• The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

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