First molecular epidemiological study of tuberculosis in Benin
Abstract:OBJECTIVES: To assess the diversity of Mycobacterium tuberculosis strains in Cotonou, Benin, and the risk factors associated with clustering.
METHODS: We analysed one sputum sample from 194 consecutive new pulmonary tuberculosis (TB) cases using two genotyping methods: spoligotyping and the 12 loci mycobacterial interspersed repetitive unit–variable number of tandem repeats (MIRU-VNTR). The data obtained were compared to the SpolDB4.0 database.
RESULTS: We have found that spoligotype 61, highly predominant in West Africa, was also the most prevalent strain in Cotonou. We observed that the Beijing family represented 10.3% of strains and was associated with resistance to streptomycin. We also confirmed that combining spoligotyping and MIRU-VNTR provided a higher discriminatory power than the two techniques used individually.
CONCLUSION: Spoligotype 61 and Beijing genotype are the most prevalent genotypes of M. tuberculosis in Cotonou.
Document Type: Regular Paper
Affiliations: 1: Laboratoire de Référence des Mycobactéries, Cotonou, Bénin; Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium 2: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium 3: Laboratoire de Référence des Mycobactéries, Cotonou, Bénin 4: Immunology Unit, Institute of Tropical Medicine, Antwerp, Belgium
Publication date: March 1, 2009
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