Drug susceptibility testing of Mycobacterium tuberculosis against second-line drugs using the Bactec MGIT 960 System
Abstract:OBJECTIVE: To establish the critical test concentrations for seven second-line anti-tuberculosis drugs in the Bactec Mycobacterial Growth Indicator Tube (MGIT) 960 TB system and to evaluate its efficacy compared to the Bactec 460 TB system.
DESIGN: This study was carried out in three phases. In Phase I, pan-susceptible strains were tested to establish the minimum inhibitory concentration; in Phase II, mostly resistant strains were tested to determine a critical test concentration; and in Phase III, actual clinical isolates were tested to validate the optimal critical concentrations established in Phases I and II.
RESULTS: The critical concentrations established for seven second-line drugs with the Bactec MGIT 960 system are amikacin 1.0 μg/ml, capreomycin 2.5 μg/ml, kanamycin 2.5 μg/ml, ofloxacin 2.0 μg/ml, moxifloxacin 1.0 μg/ml, ethionamide 5.0 μg/ml and para-amino salicylic acid 4.0 μg/ml.
CONCLUSION: The Bactec MGIT 960 System is an accurate and reliable method for rapid drug susceptibility testing (DST) of Mycobacterium tuberculosis against second-line drugs. In the present study, few of the strains were resistant to fluoroquinolones and further DST for this group is required.
Document Type: Regular Paper
Affiliations: 1: Department of Microbiology, P D Hinduja National Hospital and Medical Research Centre Tertiary Care Hospital, Mumbai, India 2: Becton Dickinson Diagnostic Systems, Becton Dickinson, Mumbai, India 3: Becton Dickinson Diagnostic Systems, Sparks, Maryland, USA
Publication date: 2008-12-01
The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.
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