Microscopic-observation drug susceptibility assay provides rapid and reliable identification of MDR-TB
Abstract:BACKGROUND: Liquid culture systems are more rapid and sensitive for both the detection and drug susceptibility testing (DST) of Mycobacterium tuberculosis.
SETTING: St Peter's TB Specialised Hospital and public health laboratory, Addis Ababa.
OBJECTIVE: To compare the microscopic-observation drug susceptibility (MODS) assay with the BACTEC-MGIT 960 system for isoniazid and rifampicin DST (i.e., multidrug-resistant tuberculosis [MDR-TB] identification) of M. tuberculosis.
DESIGN: The evaluation was based on 58 smear- and culture-positive sputum samples from patients diagnosed in Addis Ababa, Ethiopia. BACTEC-MGIT was used as the reference standard.
RESULTS: For the detection of MDR-TB, MODS has a sensitivity, specificity and accuracy rate of respectively 95%, 100% and 98.3% (κ 0.981, concordance 98.3%). Concurrent culture detection and DST results are obtained in a median of 9 days with MODS, while indirect DST results with BACTEC-MGIT are obtained in a median of 8 days (this does not include time to primary isolate).
CONCLUSION: MODS is an accurate, rapid and relatively inexpensive method for the identification of MDR-TB.
Document Type: Regular Paper
Affiliations: 1: Medical Faculty, College of Health Sciences, Hawassa University, Awassa, Ethiopia; Medical Faculty, Addis Ababa University, Addis Ababa, Ethiopia; Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia 2: Medical Faculty, Addis Ababa University, Addis Ababa, Ethiopia 3: Albert Einstein College of Medicine, Bronx, New York, New York, USA 4: Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia 5: St Peter's TB Specialised Hospital, Addis Ababa, Ethiopia
Publication date: 2008-03-01
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