Ziehl-Neelsen staining: theory and practice [Technical Note]

Authors: Van Deun, A.¹; Hossain, M.A.²; Gumusboga, M.³; Rieder, H.L.⁴

Source: The International Journal of Tuberculosis and Lung Disease, Volume 12, Number 1, January 2008 , pp. 108-110(3)

Publisher: International Union Against Tuberculosis and Lung Disease

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Abstract:

The information provided in the guidelines of the World Health Organization and the International Union Against Tuberculosis and Lung Disease for Ziehl-Neelsen staining is not practical on a number of points. The advice given here is meant to supplement the guidelines. It is based on experiments on and field experience of basic fuchsin stain and staining solutions.

Keywords: microscopy; Ziehl-Neelsen; staining; basic fuchsin

Document Type: Short communication

Affiliations: 1: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium; International Union Against Tuberculosis and Lung Disease, Paris, France 2: Damien Foundation Bangladesh, Dhaka, Bangladesh 3: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium 4: International Union Against Tuberculosis and Lung Disease, Paris, France

Publication date: 2008-01-01

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The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.
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