Beijing family Mycobacterium tuberculosis strains differ in their intracellular growth in THP-1 macrophages
OBJECTIVE: To determine whether rapid growth in macrophages is a common characteristic of Beijing family strains.
DESIGN: T-helper precursor-1 human macrophages were infected with various Beijing family strains, and intracellular growth and tumor necrosis factor alpha (TNF-α) secretion were assessed. Strains differed in their genotype, with IS6110 copy number ranging from 9 to 22.
RESULTS: Strains demonstrated a range of growth phenotypes over the 7-day infection period. Three grew significantly more slowly than the other strains, whereas the fastest growth was observed consistently with isolates of strain 210.
CONCLUSION: Rapid growth in macrophages is not a common characteristic of all Beijing strains. Few Beijing strains are as virulent as strain 210. The growth advantage is consistent with strain 210 having persisted many years in different locations and having caused many outbreaks.
Document Type: Regular Paper
Affiliations: 1: Department of Pathology, University of Arkansas for Medical Sciences and Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA 2: Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA; Louis Stokes Cleveland VA Medical Center, Cleveland, Ohio, USA; and University of Cleveland Case Medical Center, Cleveland, Ohio, USA 3: Department of Neurobiology and Developmental Sciences, University of Arkansas for Medical Sciences and Central Arkansas Veterans Healthcare System, Little Rock, Arkansas, USA
Publication date: 2007-10-01
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