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Free Content Rapid detection of Mycobacterium tuberculosis from sputum specimens using the FASTPlaqueTB test

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OBJECTIVES: To determine the performance of the FASTPlaqueTB test, based on bacteriophage amplification technology, by comparison with the BACTEC 460 TB culture system, the Löwenstein-Jensen (LJ) medium culture method and Ziehl-Neelsen (ZN) staining.

METHODS: Of 400 sputum specimens studied in our laboratory, 19 were excluded due to contaminant growth. The FASTPlaqueTB test was performed according to the manufacturer's instructions.

RESULTS: Only 42 of the 381 specimens examined were positive on at least one test: 30 were positive with ZN staining, 34 with LJ medium, 36 with the FASTPlaqueTB test and 39 with BACTEC 460 TB. The combination of BACTEC 460 TB and LJ medium culture was considered the gold standard. The sensitivity and specificity were 70.7% and 99.7% for ZN staining, 87.8% and 100% for the FASTPlaqueTB test, 82.9% and 100% for LJ, and 95.1% and 100% for BACTEC 460 TB.

CONCLUSIONS: The FASTPlaqueTB test is useful in the rapid diagnosis of TB.

Keywords: FASTPlaqueTB test; Mycobacterium tuberculosis; sputum

Document Type: Regular Paper

Affiliations: 1: Department of Microbiology, Faculty of Medicine, Eskisehir Osmangazi University, Eskisehir, Turkey 2: Microbiology Laboratory, Iskenderun State Hospital, Iskenderun, Turkey

Publication date: 2007-08-01

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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