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Free Content Evaluation of a rapid culture method on liquid Bio FM (BIO-RAD) medium for the isolation of mycobacteria

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Abstract:

SETTING: The search continues for a simple, rapid culture system for the isolation of Mycobacterium tuberculosis in low-income countries. Bio FM (BIO-RAD) medium is an enriched 7H9 medium optimised for the growth of mycobacteria and contains a chromogenic indicator.

OBJECTIVE: To evaluate the detection rate and time to detection of mycobacteria using the Bio FM system in comparison with the Löwenstein-Jensen (LJ) medium method routinely used in our laboratory.

MATERIALS AND METHODS: A total of 270 pulmonary and 178 extra-pulmonary samples were cultured in parallel on Bio FM and LJ media. The mycobacterial detection rate and time were compared.

RESULTS: The mycobacteria detection rate on Bio FM and LJ were not significantly different (respectively 97.9% and 93.15%, P > 0.05). The growth of M. tuberculosis was faster on Bio FM (mean 12.42 days [3–41] vs. 20.7 [10–48] days for LJ, P < 10−6).

CONCLUSION: In our study, the culture method on liquid Bio FM medium was faster, but the detection rate was not better than with solid LJ medium.

Keywords: Bio FM medium; rapid diagnosis; tuberculosis

Document Type: Regular Paper

Affiliations: 1: Mycobacteria Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; and National Mycobacteriology Laboratory, Tuberculosis and Leprosy Service, Ministry of Health and Family Planning, Antananarivo, Madagascar 2: Mycobacteria Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar 3: Department of Chest Diseases (Pavillon Roques), Centre Hospitalier de Soavinandriana, Antananarivo, Madagascar 4: Epidemiology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar

Publication date: 2007-08-01

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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