Evaluation of a rapid culture method on liquid Bio FM (BIO-RAD) medium for the isolation of mycobacteria
Abstract:SETTING: The search continues for a simple, rapid culture system for the isolation of Mycobacterium tuberculosis in low-income countries. Bio FM (BIO-RAD) medium is an enriched 7H9 medium optimised for the growth of mycobacteria and contains a chromogenic indicator.
OBJECTIVE: To evaluate the detection rate and time to detection of mycobacteria using the Bio FM system in comparison with the Löwenstein-Jensen (LJ) medium method routinely used in our laboratory.
MATERIALS AND METHODS: A total of 270 pulmonary and 178 extra-pulmonary samples were cultured in parallel on Bio FM and LJ media. The mycobacterial detection rate and time were compared.
RESULTS: The mycobacteria detection rate on Bio FM and LJ were not significantly different (respectively 97.9% and 93.15%, P > 0.05). The growth of M. tuberculosis was faster on Bio FM (mean 12.42 days [3–41] vs. 20.7 [10–48] days for LJ, P < 10−6).
CONCLUSION: In our study, the culture method on liquid Bio FM medium was faster, but the detection rate was not better than with solid LJ medium.
Document Type: Regular Paper
Affiliations: 1: Mycobacteria Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar; and National Mycobacteriology Laboratory, Tuberculosis and Leprosy Service, Ministry of Health and Family Planning, Antananarivo, Madagascar 2: Mycobacteria Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar 3: Department of Chest Diseases (Pavillon Roques), Centre Hospitalier de Soavinandriana, Antananarivo, Madagascar 4: Epidemiology Unit, Institut Pasteur de Madagascar, Antananarivo, Madagascar
Publication date: 2007-08-01
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