IngentaConnect Study of M. tuberculosis ES-31 and ES-20 antigen levels in differ...

Authors: Shende, N.¹; Upadhye, V.¹; Kumar, S.²; Gangane, N.³; Harinath, B.C.¹

Source: The International Journal of Tuberculosis and Lung Disease, Volume 11, Number 2, February 2007 , pp. 222-226(5)

Publisher: International Union Against Tuberculosis and Lung Disease

Abstract:

OBJECTIVE: To study tuberculous excretory-secretory (ES) 31 and ES-20 antigens in different pathogenic grades of lymph node tuberculosis (TB).

DESIGN: The study group included lymph node TB patients showing granuloma with mature epithelioid cells based on cytology findings (strong immune response group, SI) and patients showing no granuloma formation and acellular necrosis (weak immune response group, WI). Sandwich ELISA was performed using affinity purified antibodies against Mycobacterium tuberculosis ES-31 and ES-20 antigens to assay free and immune complexed antigen levels in the serum of these patients.

RESULTS: Higher levels of immune complexed ES-31 (geometric mean titre [GMT] 848) and ES-20 (GMT 1818) antigens than free ES-31 (GMT 462) and ES-20 (GMT 647) were observed in WI patients. There were higher levels of immune complexed ES-20 antigen levels (GMT 1818) in WI patients than in SI lymph node TB patients; the difference was significant (P < 0.05).

CONCLUSION: Elevated levels of immune complexed ES-20 antigen in patient's serum may be a useful immunological marker for weak immune response patients in lymph node TB.

Keywords: ELISA; immune complexes; lymph node TB

Document Type: Regular paper

Affiliations: 1: Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India 2: Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India 3: Department of Pathology, Mahatma Gandhi Institute of Medical Sciences, Sevagram, Wardha, India

Study of M. tuberculosis ES-31 and ES-20 antigen levels in different pathogenic grades of lymph node tuberculosis