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Free Content Light emitting diodes for auramine O fluorescence microscopic screening of Mycobacterium tuberculosis [Technical Note]

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We describe the simple adaptation of a standard fluorescent microscope for illumination using a ‘Royal Blue’ Luxeon™ light emitting diode (LED) and demonstrate that this form of illumination is suitable for the detection of auramine O stained Mycobacterium spp. The low cost, low power consumption, safety and reliability of LEDs makes them attractive alternatives to mercury vapour lamps.

Keywords: LED; auramine; fluorescence; microscope

Document Type: Short Communication

Affiliations: Department of Biomedical Research, Royal Tropical Institute (KIT), Amsterdam, The Netherlands

Publication date: 2006-09-01

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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