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Free Content Real-time PCR assay for improved detection of Mycobacterium tuberculosis complex in paraffin-embedded tissues [Short Communication]

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Abstract:

OBJECTIVE: To test the usefulness of a commercially available real-time polymerase chain reaction (PCR) kit for the detection of Mycobacterium tuberculosis complex (MTBC) in formalin-fixed, paraffin-embedded tissues.

RESULTS: The examination of 24 specimens of patients with a final diagnosis of TB shows that the real-time PCR assay exhibits a higher sensitivity (66.7%) for the detection of MTBC DNA than an alternative in-house IS6110 PCR (33.3%), whereas staining detected acid-fast bacilli in only two cases (8.3%).

CONCLUSION: The real-time PCR assay provides a highly sensitive and specific means for the detection of MTBC DNA in histopathological specimens.

Keywords: Mycobacterium tuberculosis complex; formalin fixation; real-time PCR

Document Type: Short Communication

Affiliations: 1: National Reference Center for Mycobacteria, Research Center Borstel, Borstel, Germany 2: Clinical and Experimental Pathology, Research Center Borstel, Borstel, Germany

Publication date: March 1, 2006

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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