@article {Van Deun:2005:1027-3719:1127,
title = "Performance of variations of carbolfuchsin staining of sputum smears for AFB under field conditions",
journal = "The International Journal of Tuberculosis and Lung Disease",
parent_itemid = "infobike://iuatld/ijtld",
publishercode ="iuatld",
year = "2005",
volume = "9",
number = "10",
publication date ="2005-10-01T00:00:00",
pages = "1127-1133",
itemtype = "ARTICLE",
issn = "1027-3719",
eissn = "1815-7920",
url = "https://www.ingentaconnect.com/content/iuatld/ijtld/2005/00000009/00000010/art00010",
keyword = "Ziehl-Neelsen, staining, microscopy, carbolfuchsin",
author = "Van Deun, A. and Hamid Salim, A. and Aung, K. J. M. and Hossain, Md. A. and Chambugonj, N. and Hye, Md. A. and Kawria, A. and Declercq, E.",
abstract = "SETTING: A field project in Bangladesh.OBJECTIVE: To compare the effectiveness of commonly used carbolfuchsin staining variations.DESIGN: Routine hot Ziehl-Neelsen (ZN) 1% basic fuchsin staining for 15 min in 75 field clinics. Blind reading of duplicate smears stained by ZN 1% vs. 0.3% basic fuchsin applied for 5 min, or by ZN 1% 5 min vs. Kinyoun cold staining. Rechecking of discordant series.RESULTS: For comparable numbers of false positives, sensitivity was significantly lower with Kinyoun than with ZN 1% 5 min (85.6% vs. 93.0%, P CONCLUSIONS: Kinyoun cold staining sensitivity was unsatisfactory in field clinics. The sensitivity of the WHO/IUATLD recommended 0.3% fuchsin for 5 min was not significantly different from the original 1% ZN for 5 min, but 1% 15 min hot staining might be superior. A reduced fuchsin concentration together with a short staining time may leave too narrow a margin for error. TB programmes using hot ZN with a concentrated stain or longer staining time should not be urged to change.",
}