Mutations responsible for Mycobacterium tuberculosis isoniazid resistance in Italy
OBJECTIVE: To investigate the mutations responsible for INH resistance among Italian isolates of M. tuberculosis, to assess the feasibility of predicting drug resistance using a genetic approach.
DESIGN: The mutations responsible for INH resistance were looked for in selected regions of genes katG, kasA and ndh and in the promoter regions of inhA and ahpC by nucleotide sequencing, and the results were compared with data reported in other studies.
RESULTS: Prevalent INH resistance mutations were found at codon 315 of the katG gene and at position −15 of the inhA regulatory region (respectively 37.8% and 20.0% of isolates). The prevalence of mutations at position −24 of inhA, in ahpC, and in kasA ranged from 2.2% to 4.4%. No mutations were found in 35.6% of the isolates.
CONCLUSION: The identification of INH resistance by genetic analysis of the selected regions may be inappropriate in areas with a low prevalence of TB, such as Italy, as the genetic mechanisms of resistance remain unidentified for approximately one third of the isolates.
Document Type: Regular Paper
Affiliations: 1: Dipartmento di Patologia Sperimentale, Biotecnologie Mediche, Infettivologia ed Epidemiologia, Università di Pisa, Pisa, Italy 2: Centro Regionale di Riferimento per i Micobatteri, Laboratorio di Microbiologia e Virologia, Ospedale Careggi, Florence, Italy
Publication date: 2005-01-01
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