Simple, phage-based (FASTPlaque) technology to determine rifampicin resistance of Mycobacterium tuberculosis directly from sputum
Source: The International Journal of Tuberculosis and Lung Disease, Volume 8, Number 9, September 2004 , pp. 1114-1119(6)
Abstract:SETTING: Cape Town, South Africa.
OBJECTIVE: To evaluate the performance of a simple, manual, phage-based test for determining rifampicin (RMP) resistance of Mycobacterium tuberculosis directly from smear-positive sputum specimens.
DESIGN: A comparative study of the performance of the FASTPlaque (phage amplification) technology to determine RMP resistance directly from smear-positive sputum compared with isolation and the conventional indirect Middlebrook 7H11 agar proportion method.
RESULTS: The FASTPlaque direct RMP test achieved sensitivity, specificity and overall accuracy of 100% (11/11), 100% (134/134) and 100% (145/145), respectively, compared with the conventional indirect susceptibility test method (resolved data). The FASTPlaque direct RMP test reported results within 2 days from receipt of the specimen, while the conventional method took between 27 and 103 days (mean ± SD 33.2 ± 7.2 days).
CONCLUSION: FASTPlaque technology applied directly to smear-positive sputum offers performance comparable to conventional methods, with results available in 2 days instead of weeks to months. The test may form a useful part of DOTS-Plus programmes to combat multidrug-resistant tuberculosis, improving patient prognosis and reducing ongoing transmission of disease. It does not require specialised equipment, making it appropriate for high-burden countries.
Document Type: Regular Paper
Publication date: 2004-09-01
- The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.
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