Free Content Evaluation of a new automated, rapid, colorimetric culture system using solid medium for laboratory diagnosis of tuberculosis and determination of anti-tuberculosis drug susceptibility

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Abstract:

SETTING: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey, a tertiary referral hospital in a region endemic for tuberculosis.

OBJECTIVE: To evaluate the performance of the Dio-TK Culture System (CS), a new, rapid, automated colorimetric culture system.

DESIGN: Dio-TK CS results were compared with routinely used Löwenstein Jensen (LJ) medium and Bactec 460 TB.

RESULTS: In this study, 449 specimens, mostly sputum samples obtained from 348 patients, were evaluated. Mycobacteria were isolated from 31 (6.9%), 23 (5.1%), 18 (4.0%) and 21 (4.7%) of the specimens using Bactec 12B, LJ, Dio-TK Medium and Dio-TK SLC (selective), respectively. The mean time to detection of growth of 13 isolates by Bactec 12B, Dio-TK Medium, Dio-TK SLC and LJ medium was respectively 8.9, 15.1, 17.0 and 26.1 days.

CONCLUSION: Dio-TK may be a practical and rapid culture system for daily use. However, the manufacturer should improve the system to minimise the effects of manipulation errors. Comparative studies with a larger number of isolates are needed to standardise drug concentrations used in anti-tuberculosis drug susceptibility testing.

Keywords: Bactec 460 TB; Dio-TK Culture System; Löwenstein Jensen medium; tuberculosis

Document Type: Regular Paper

Affiliations: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey

Publication date: June 1, 2004

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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