Evaluation of a new automated, rapid, colorimetric culture system using solid medium for laboratory diagnosis of tuberculosis and determination of anti-tuberculosis drug susceptibility
Abstract:SETTING: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey, a tertiary referral hospital in a region endemic for tuberculosis.
OBJECTIVE: To evaluate the performance of the Dio-TK Culture System (CS), a new, rapid, automated colorimetric culture system.
DESIGN: Dio-TK CS results were compared with routinely used Löwenstein Jensen (LJ) medium and Bactec 460 TB.
RESULTS: In this study, 449 specimens, mostly sputum samples obtained from 348 patients, were evaluated. Mycobacteria were isolated from 31 (6.9%), 23 (5.1%), 18 (4.0%) and 21 (4.7%) of the specimens using Bactec 12B, LJ, Dio-TK Medium and Dio-TK SLC (selective), respectively. The mean time to detection of growth of 13 isolates by Bactec 12B, Dio-TK Medium, Dio-TK SLC and LJ medium was respectively 8.9, 15.1, 17.0 and 26.1 days.
CONCLUSION: Dio-TK may be a practical and rapid culture system for daily use. However, the manufacturer should improve the system to minimise the effects of manipulation errors. Comparative studies with a larger number of isolates are needed to standardise drug concentrations used in anti-tuberculosis drug susceptibility testing.
Document Type: Regular Paper
Affiliations: Department of Microbiology and Clinical Microbiology, Gulhane Military Medical Academy, Ankara, Turkey
Publication date: 2004-06-01
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