Free Content Drug susceptibility testing of Mycobacterium tuberculosis to fluoroquinolones: first experience with a quality control panel in the Nordic-Baltic collaboration

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Abstract:

In the first attempt to establish a quality assurance programme for susceptibility testing of Mycobacterium tuberculosis to fluoroquinolones, 20 strains with different fluoroquinolone susceptibility patterns were distributed by the Supranational Reference Laboratory in Stockholm to the other mycobacterial reference laboratories of the Nordic and Baltic countries. Susceptibility testing to fluoroquinolones was performed according to routine procedures in each laboratory. Results were compared to sequence analysis of the gyrA gene and minimal inhibitory concentration determination. Most laboratories found identical susceptibility patterns. The two resistant strains were correctly identified by all laboratories, but three laboratories each falsely reported one susceptible strain as resistant. These results indicate that the participating laboratories yield reliable results in detection of fluoroquinolone-resistant strains, although the need for a standardised quality assurance programme for drug susceptibility testing for fluoroquinolones is stressed by the strains falsely reported as resistant.

Keywords: MDR-TB; quality assurance; second-line drugs

Document Type: Short Communication

Affiliations: 1: International Reference Laboratory of Mycobacteriology, Copenhagen, Denmark 2: Department of Microbiological R&D, Statens Serum Institut, Copenhagen, Denmark 3: Division of Infectious Diseases Control, Institute of Public Health, Oslo, Norway 4: Department of Clinical Microbiology, Karolinska Institute and Hospital, Stockholm, Sweden 5: Mycobacterial Reference Laboratory, National Public Health Institute, Turku, Finland 6: Mycobacteriology Laboratory, Kivimae Hospital, Tallinn, Estonia 7: National Tuberculosis Reference Laboratory, National Tuberculosis and Lung Diseases Hospital, Vilnius, Lithuania 8: State Centre of Tuberculosis and Lung Diseases, Riga, Latvia 9: Department of Bacteriology, Swedish Institute for Infectious Diseases Control, Solna, Sweden

Publication date: September 1, 2003

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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