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Free Content Standardisation and evaluation of DNA-lanthanide fluorescence spectroscopy for determining rifampicin resistance in Mycobacterium tuberculosis clinical isolates

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SETTING: Tuberculosis Research Centre, Chennai.

OBJECTIVE: To rapidly identify multidrug-resistant Mycobacterium tuberculosis using a novel method.

DESIGN: A new assay, based on DNA-lanthanide fluorescence, was standardised and evaluated using 93 each of coded rifampicin-resistant and rifampicin-sensitive M. tuberculosis clinical isolates for the correct identification of rifampicin resistance. The results obtained by the new assay were compared with the conventional results.

RESULTS AND CONCLUSION: The new assay gave a sensitivity and specificity of 88% and 85%, respectively. It is simple, easy to perform and requires 48 hours for the drug susceptibility results to be available after obtaining the primary culture.

Keywords: M. tuberculosis; early detection; rifampicin resistance

Document Type: Regular Paper

Affiliations: 1: Tuberculosis Research Centre (Indian Council of Medical Research) Chennai, India 2: Indira Gandhi Centre for Atomic Radiation, Kalpakkam, Chennai, India

Publication date: September 1, 2003

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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