Rapid and simple MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis

Authors: Foongladda S.¹; Roengsanthia D.¹; Arjrattanakool W.¹; Chuchottaworn C.²; Chaiprasert A.¹; Franzblau S. G³

Source: The International Journal of Tuberculosis and Lung Disease, Volume 6, Number 12, December 2002 , pp. 1118-1122(5)

Publisher: International Union Against Tuberculosis and Lung Disease

Abstract:

The MTT method for rifampicin and isoniazid susceptibility testing of Mycobacterium tuberculosis was developed by using bacterial suspension prepared from colonies on solid media. The MTT tube assay in 1 ml Middlebrook 7H9 broth was completed within 4 days for rifampicin (RMP) and within 7 days for isoniazid (INH). When MTT assay results with 279 M. tuberculosis clinical isolates were compared with those of the conventional proportion method on Löwenstein-Jensen medium, high specificity and sensitivity values of 100% and 94.1%, respectively, for RMP susceptibility testing, and 99.5% and 89.2%, respectively, for INH susceptibility testing were obtained. The accuracy of the MTT method for RMP and INH was >0.97 concordance with the proportion method. The MTT method is simple, inexpensive and rapid. The high level of agreement with the conventional proportion method suggests a potential to rapidly detect drug-resistant M. tuberculosis in developing countries, as only basic microbiological equipment is need.

Keywords: M. tuberculosis; susceptibility testing; MTT

Language: English

Document Type: Short communication

Affiliations: 1: Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand 2: Central Chest Hospital, Department of Communicable Disease Control, Ministry of Public Health, Nonthaburi, Thailand 3: Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois, USA

Publication date: 2002-12-01

• The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

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