Rapid molecular diagnosis of tuberculous meningitis using the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test in a large Canadian public health laboratory
RESULTS: In total, 311 CSF specimens were tested by the MTD, of which 17 were positive. When compared with culture (gold standard), the sensitivity and specificity of the MTD test were 93.8% and 99.3%, respectively. The positive and negative predictive values for TBM were 88.2%, and 99.7%. Clinical and epidemiological information was requested for all culture-positive TBM patients. These data were used to assess the mortality rate (55.6%) and to determine common factors that could be applied as selection criteria for the appropriate testing of CSF by MTD.
CONCLUSION: The study found the MTD test to be a rapid, sensitive and specific test for TBM. A history of immigration from an area endemic for tuberculosis (TB), a history of TB, symptoms of neurological deficits and the results of CSF analyses could be used to appropriately select CSF for MTD testing in order to provide a critical early diagnosis of TBM.
Document Type: Regular Paper
Affiliations: Clinical and Environmental Microbiology, Laboratories Branch, Ontario, Ministry of Health and Long-Term Care, Etobicoke, Ontario, Canada
Publication date: 2002-10-01
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