Free Content Variations in the NRAMP1 gene and susceptibility of tuberculosis in Taiwanese

 Download
(PDF 143.8 kb)
 
Download Article:

Abstract:

SETTING: National Taiwan University Hospital, Taipei, Taiwan.

OBJECTIVE: To study the variations in the NRAMP1 gene using five genotypes (274C/T, 577-18G/A, A318V, D543N and 3′ untranslated region [UTR]), and the susceptibility of tuberculosis and HIV infection in Taiwanese.

DESIGN: The study sample included 49 patients with tuberculosis, 48 healthy control subjects and 60 HIV-infected patients. The polymerase chain reaction (PCR) products amplified from their genomic DNA were subjected to restriction enzyme digestion and were analysed using agarose gel electrophoresis.

RESULTS: A318V was not polymorphic in the studied population. Only D543N and 3′UTR were more heterozygous. In 274 C/T and 577-18G/A, the allele frequencies showed the predominant type to be the homozygous patterns C/C (94%) and G/G (94%), respectively. There were no statistically significant differences between the tuberculosis patients and the healthy control subjects. Despite the high susceptibility to Mycobacterium tuberculosis in HIV-infected patients, genotypic frequencies in the HIV-positive patients were not significantly different between tuberculous (n = 29) and non-tuberculous patients (n = 31). In comparison with previous studies, there were significant differences between different ethnic groups in allele frequencies for 274C/T, D543N and 3′UTR.

CONCLUSION: The allele and genotype of NRAMP1 polymorphism among Taiwanese differed from those of Caucasians, Africans and Hispanics. No allelic associations were identified between the NRAMP1 alleles and tuberculosis susceptibility.

Keywords: HIV infection; NRAMP1; Taiwan; susceptibility; tuberculosis

Document Type: Regular Paper

Affiliations: 1: Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan 2: Department of Clinical Research, College of Medicine, National Taiwan University, Taipei, Taiwan 3: Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan 4: Department of Medical Technology, Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan 5: Department of Laboratory Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan

Publication date: May 1, 2002

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more