Washing of new microscopic glass slides in dichromate solution does not influence sputum AFB smear results [Notes from the Field]
Abstract:SETTING: Microscopy centres in Tiruvallur District, Tamil Nadu, India, implementing DOTS.
OBJECTIVE: To know whether washing new glass slides in dichromate solution is essential for effective sputum acid-fast bacilli microscopy.
METHODS: Two direct smears were prepared from each of 1750 sputum samples. One was made on dichromate solution-cleaned new glass slides and the other was made on unwashed new glass slides. The smears were blinded and examined.
RESULTS: Of the 1750 specimens, 13.5% and 13.08% were positive for AFB using washed and unwashed slides, respectively (P = 0.12). The concordance between these two (including one grade above and one below) was 98.7%.
CONCLUSION: Washing of new glass slides in dichromate solution is not essential for AFB microscopy.
Document Type: Short Communication
Publication date: 2002-03-01
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