Rifampin and isoniazid resistance associated mutations in Mycobacterium tuberculosis clinical isolates in Seville, Spain
Abstract:The susceptibility phenotypes of 964 clinical isolates of Mycobacterium tuberculosis were studied over a 7-year period in Seville, Spain. Thirty-eight (3.9%) strains were rifampin (RMP) resistant, 79 (8.2%) were isoniazid (INH) resistant and 22 (2.3%) were resistant to at least both antimicrobials (multidrug-resistant, MDR). We studied the mechanisms of resistance to these drugs in 94 resistant clinical isolates of M. tuberculosis using three molecular methods: 1) PCR-single strand conformation polymorphism (SSCP) analysis, 2) RFLP analysis using B1/B2 primers, and 3) sequence analysis. Five different mutations were detected in the rpoB gene: Ser531→Leu (72.3%), His526→Asp (12.8%), Asn518→Ser (2.1%), Gln513→Leu (2.1%) and a nine-nucleotide deletion (2.1%). In the case of resistance to INH, four different mutations in the katG gene were detected, Ser315→Thr (58.0%), Ser315→Leu (2.9%), partial deletion (5.8%) and Ile304→Val (1.4%), while in the inhA regulatory region the only mutation was the nucleotide substitution C209T (4.3%). No mutation was found in the ahpC promoter.
Document Type: Short Communication
Affiliations: 1: Department of Microbiology, University of Seville School of Medicine, Seville, Spain 2: Department of Microbiology, University of Seville School of Medicine, Seville, Spain and Hospital Universitario Virgen Macarena, University of Seville, Seville, Spain 3: Department of Microbiology, University of Seville School of Medicine, Seville, Spain and Hospital Universitario Virgen del Rocío, University of Seville, Seville, Spain
Publication date: February 1, 2002
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