Free Content Molecular and conventional epidemiology of tuberculosis in an inner city district

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Abstract:

SETTING: Molecular epidemiology has underlined the importance of recent tuberculosis (TB) transmission and has uncovered notable discrepancies compared with conventional epidemiology.

OBJECTIVES: 1) To determine, by RFLP analysis, the percentage of clustered cases in an inner city district with a high incidence of TB (163/100000) and the groups at risk of being clustered; and 2) to compare the role of conventional contact tracing (CCT) with that of RFLP.

DESIGN: RFLP was carried out using the IS6110 and pTBN12 (PGRS) sequences of 165 cultures positive for Mycobacterium tuberculosis in the Ciutat Vella district of Barcelona during 1997–1998. Contact tracing was carried out in 171 of 251 declared cases (68.1%). Associations were assessed by calculating odds ratios (OR) with 95% confidence intervals (CI). Logistic regression was used for multivariate analysis.

RESULTS: Using RFLP, 76 (46.0%) strains were found to be clustered by IS6110 and PGRS. From CCT, 30 new patients were found among 858 contacts (3.5%) and 57 patients were linked. In terms of RFLP and CCT, the main risk factor was intravenous drug use (IVDU). In 44 cases who lived alone and were not involved in CCT, 50% were in RFLP clusters. The concordance rate between RFLP and CCT was 8/13 (61.5%); the disagreement corresponded to a cluster of five recent immigrants from Africa. Subsequent to RFLP, an epidemiological connection was found in 15/55 cases (27.2%).

CONCLUSIONS: The percentage of clustered cases is very high. CCT was useful for identifying new cases, but it was insufficient for detecting the pathways of transmission. CCT coverage needs to be improved in marginalized individuals, and the results correlated with those of RFLP.

Keywords: RFLP; conventional contact tracing (CCT); epidemiology; tuberculosis (TB)

Document Type: Regular Paper

Affiliations: 1: Centro de Prevención y Control de la Tuberculosis ‘Dr Lluís Sayé’, Barcelona, Spain 2: Servicio de Epidemiologia. Instituto Municipal de la Salud, Barcelona, Spain 3: Hospital de San Pau, Servei de Microbiologia, Barcelona, Spain 4: Dirección de Asistencia Primària (DAP) de Ciutat Vella (Barcelona), Áreas Básicas de Salud (ABS) ‘Raval Nord’, ‘Raval Sud’, ‘Casc Antic’, ‘Gòtic’, ‘Lluís Sayé’ y ‘Barceloneta’, Barcelona, Spain 5: Hospital de San Pau, Servei de Microbiologia, Barcelona, Spain and Departament de Genética i Microbiologia, Universidad Autònoma de Barcelona, Barcelona, Spain 6: Barcelona, Spain

Publication date: August 1, 2001

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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