Spacer oligonucleotide typing of bacteria of the Mycobacterium tuberculosis complex: recommendations for standardised nomenclature [The Language of Our Science]
Authors: Dale J. W.1; Brittain D.2; Cataldi A. A.3; Cousins D.4; Crawford J. T.5; Driscoll J.6; Heersma H.7; Lillebaek T.8; Quitugua T.9; Rastogi N.10; Skuce R. A.2; Sola C.10; Van Soolingen D.7; Vincent V.11
Source: The International Journal of Tuberculosis and Lung Disease, Volume 5, Number 3, March 2001 , pp. 216-219(4)
Publisher: International Union Against Tuberculosis and Lung Disease
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Abstract:
Spacer oligonucleotide typing (spoligotyping) is widely used for differentiation of bacteria of the Mycobacterium tuberculosis complex. However, the absence of any standardised method for concise description of spoligotypes makes it difficult to compare the results from different laboratories. This paper describes unambiguous, interconvertible systems for the designation of spoligotype patterns, the adoption of which will be beneficial to mycobacterial research.
Keywords: Mycobacterium tuberculosis; spoligotyping; epidemiology
Language: English
Document Type: Invited paper
Affiliations: 1: School of Biological Sciences, University of Surrey, Guildford, Surrey, UK 10: Tuberculosis & Mycobacteria Unit, Institut Pasteur de Guadeloupe, Morne Jolivière, Pointe-à-Pitre, Guadeloupe 11: Laboratoire de Référence des Mycobactéries, Institut Pasteur, Paris, France 2: Veterinary Sciences Division, Department of Agriculture and Rural Development, Stormont, Belfast, Northern Ireland 3: Instituto de Biotecnología, CICVyA- INTA, Los Reseros y Las Cabañas, Hurlingham, Argentina 4: Australian Reference Laboratory for Bovine Tuberculosis, Agriculture Western Australia, South Perth, Australia 5: Centers for Disease Control and Prevention, Atlanta, Georgia 6: Wadsworth Center, New York State Department of Health, Albany, New York 7: Rijksinstituut voor Volksgezondheid en Milieuhygiene, Bilthoven, Netherlands 8: International Reference Laboratorium for Mycobacteriology, Statens Serum Institut, Copenhagen, Denmark 9: Molecular Mycobacteriology Laboratory, Department of Clinical Investigation, Texas Center for Infectious Disease, San Antonio, Texas
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