Skip to main content

Free Content DNA fingerprinting of a national sample of Mycobacterium tuberculosis isolates, Botswana, 1995–1996

Download Article:
(PDF 227.7 kb)
DNA fingerprinting may be useful to elucidate tuberculosis (TB) transmission in community settings, but its utility is limited if only few fingerprint patterns are observed or band numbers are low. We performed DNA fingerprinting on a national, population-based sample of Mycobacterium tuberculosis isolates from Botswana. During 1995–1996, a random sample of 213 isolates, representing 5% of all smear-positive TB cases, underwent DNA fingerprinting using restriction fragment length polymorphism (RFLP) IS6110 analysis. Eighty-two (38%) of the 213 isolates belonged to one of 18 clusters, with 2–9 isolates/cluster. The median number of bands was 10 (range 1–19); 183 (86%) had six or more bands. Sixty-three (49%) of 128 patients tested were infected with the human immunodeficiency virus (HIV). The degree of RFLP pattern heterogeneity and high band number support the feasibility of a prospective DNA fingerprinting study in Botswana.
No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: Africa; Botswana; DNA fingerprinting; RFLP; tuberculosis

Document Type: Short Communication

Affiliations: 1: Division of TB Elimination, National Centers for HIV/AIDS, STD and TB Prevention, Atlanta, Georgia, USA; and Epidemiology Program Office, Atlanta, Georgia, USA 2: Division of AIDS, STD and Tuberculosis Laboratory Research, Centers for Disease Control and Prevention, Atlanta, Georgia, USA 3: Mycobacteriology Laboratory, National Health Laboratories, Gaborone, Botswana 4: BOTUSA TB Project, Gaborone, Botswana 5: Division of TB Elimination, National Centers for HIV/AIDS, STD and TB Prevention, Atlanta, Georgia, USA 6: Communicable Diseases Section, Botswana Ministry of Health, Gaborone, Botswana

Publication date: 01 June 2000

More about this publication?
  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • International Journal of Tuberculosis and Lung Disease
  • Public Health Action
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more