Detection of rifampicin resistance in Mycobacterium tuberculosis isolates from diverse countries by a commercial line probe assay as an initial indicator of multidrug resistance [Technical Note]
Abstract:The line probe assay (LiPA), a rapid molecular method for detecting rifampicin resistance (RMP r) in Mycobacterium tuberculosis, correctly identified all 145 rifampicin-sensitive (RMP s) and 262 (98.5%) of 266 RMP r strains among 411 isolates collected from diverse countries. If used as a marker of multidrug-resistant tuberculosis (MDR-TB), detection of RMP r by LiPA would have detected 236 of the 240 MDR strains in this study but would have wrongly suggested the presence of MDR in 26 RMP-monoresistant isolates (sensitivity 98.3%, specificity 84.8%). Hence, the reliability of using LiPA (or any other rapid RMP r-detection method) as a surrogate marker of MDR-TB largely depends on the prevalence of RMP-monoresistance in the study population. This approach must therefore be validated in each local situation.
Document Type: Short Communication
Affiliations: Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium
Publication date: 2000-05-01
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