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Restriction fragment length polymorphism analysis of Mycobacterium avium isolates from animal and human sources

Authors: O'Grady, D.1; Flynn, O.1; Costello, E.1; Quigley, F.1; Gogarty, A.1; McGuirk, J.1; O'Rourke, J.1; Gibbons, N.2

Source: The International Journal of Tuberculosis and Lung Disease, Volume 4, Number 3, March 2000 , pp. 278-281(4)

Publisher: International Union Against Tuberculosis and Lung Disease

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Restriction fragment length polymorphism (RFLP) analysis using probes derived from the insertion sequences IS901, IS1245 and IS1311, was carried out on Mycobacterium avium isolates obtained from 18 human patients, 44 deer, 14 pigs and five cattle in the Republic of Ireland. Forty-two of the cervine isolates and two of the bovine isolates contained IS901, while this insertion sequence was absent from all of the human and porcine isolates. RFLP analysis with IS901 probe differentiated the 44 field isolates which contained this element into three types. All of the IS901-positive isolates had a characteristic three-band IS1245 hybridisation pattern and a characteristic single-band IS1311 hybridisation pattern. The IS901-negative isolates exhibited highly polymorphic IS1245 and IS1311 hybridisation patterns which differentiated the human and porcine isolates into a wide diversity of strain types.

Keywords: DNA fingerprinting; Mycobacterium avium; RFLP

Document Type: Short Communication

Affiliations: 1: Central Veterinary Research Laboratory, Abbotstown, Castleknock, Ireland 2: St. James's Hospital, Dublin, Ireland

Publication date: March 1, 2000

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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