Sensitivity and specificity of a multi-antigen ELISA test for the serological diagnosis of tuberculosis [Technical Note]
Abstract:A new serological assay (Detect-TB, BioChem ImmunoSystems), using three recombinant proteins and two synthetic peptides for the detection of the anti-Mycobacterium tuberculosis IgG, was evaluated using a panel of serum specimens collected from 100 tuberculosis (TB) patients and 270 controls, in comparison with a home-made ELISA test using purified protein derivative (PPD) as antigen. Detect-TB presented a higher sensitivity (75%) compared to PPD-ELISA (56%; P < 0.01), while the specificity of each assay was similar (Detect-TB 97%; PPD-ELISA 100%; P > 0.80). Receiver operating characteristics (ROC) analysis obtained with these data confirmed the higher level of performance of Detect-TB in comparison with PPD-ELISA. Considering the rapidity, cost-effectiveness and simplicity of this assay, its use may provide useful clinical information aiding in the rapid diagnosis of difficult TB cases.
Document Type: Short Communication
Affiliations: 1: Department of Medical Sciences, Section of Respiratory Medicine, University of Modena, Modena, Italy; and Laboratory of Immunochemistry and Molecular Pathology, Department of Biology, University ‘Tor Vergata’, Rome, Italy 2: BioChem ImmunoSystems Inc., Montreal, Quebec, Canada 3: Medical Services, ‘S. Anna’ Correctional facility, Modena, Italy 4: Department of Medical Sciences, Section of Respiratory Medicine, University of Modena, Modena, Italy
Publication date: 1999-08-01
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