In vitro susceptibility of Mycobacterium kansasii to the difluorinated quinolone sparfloxacin using a broth microdilution and macrodilution MIC system
DESIGN: The MICs were determined by two methods: broth microdilution in microplates and broth macrodilution in tubes. The isolates were inoculated into two-fold drug dilutions (ranging from 0.063 to 8 μg/ml) in Middlebrook 7H9 broth and then incubated at 37°C for 21 days.
RESULTS: All 32 strains were susceptible, with identical MIC results in both methods, 96.9% of them showing an MIC of 0.25 μg/ml.
CONCLUSION: These MIC studies suggest that sparfloxacin may be useful for drug treatment of slow-growing nontuberculous mycobacteria such as M. kansasii. The microdilution method appears to be a reliable method for routine susceptibility testing of M. kansasii, and is easy to interpret and to carry out.
Document Type: Regular Paper
Affiliations: Servicio de Microbiología y Parasitología, Hospitals Vall d'Hebron. Autonomous University, Barcelona, Spain
Publication date: 1999-04-01
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