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Free Content Quality control of smear microscopy for acid-fast bacilli: the case for blinded re-reading

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Abstract:

SETTING: Quality control of sputum smear microscopy, which is essential for ensuring correct tuberculosis (TB) diagnosis, is often performed through the unblinded re-reading of all positive slides and a sample of negative slides.

OBJECTIVE: To assess misclassification error introduced by knowledge of prior results.

METHODS: The Southern Vietnam Regional TB Laboratory prepared three gold-standard sets of 750 slides: an unblinded set, an unblinded set in which 13% of negative slides were replaced by weakly positive slides purposefully mislabelled as negative, and a blinded set. Six provincial technicians who normally perform district quality control each reread 125 slides from each set.

RESULTS: In the three sets only one negative slide was misread as positive. In the unblinded set (referent), 2.9% (9/311) positive slides were misread as negative, compared with 18.7% (57/305) in the blinded set (prevalence ratio [PR] = 6.5; 95% confidence interval [CI] 3.3–12.8; P < 0.001), and 11.3% (33/293) in the unblinded set with mislabelled slides (PR = 3.9; 95%CI 1.9–8.0; P < 0.001).

CONCLUSIONS: False-negative error was more common than false-positive error. Knowledge of prior reading influences re-reading. Blinded re-reading of systematically selected slides would appear preferable, although this method requires high levels of proficiency among quality control technicians.

Keywords: Vietnam; acid-fast bacillus; microscopy; quality control; tuberculosis

Document Type: Regular Paper

Affiliations: 1: Pham Ngoc Thach TB and Lung Disease Centre, Ho Chi Minh City, Vietnam 2: Division of Tuberculosis Elimination, National Center for HIV, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia, USA 3: National Institute of Tuberculosis and Respiratory Diseases, Hanoi, Vietnam

Publication date: 1999-01-01

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  • The International Journal of Tuberculosis and Lung Disease publishes articles on all aspects of lung health, including public health-related issues such as training programmes, cost-benefit analysis, legislation, epidemiology, intervention studies and health systems research. The IJTLD is dedicated to the continuing education of physicians and health personnel and the dissemination of information on tuberculosis and lung health world-wide.

    Certain IJTLD articles are selected for translation into French, Spanish, Chinese or Russian. They are available on the Union website

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