Evaluation of Roche Amplicor PCR assay for Mycobacterium avium complex in bronchial washing
Authors: Matsumoto, H.; Tsuyuguchi, K.; Suzuki, K.; Tanaka, E.; Amitani, R.; Kuze, F.
Source: The International Journal of Tuberculosis and Lung Disease, Volume 2, Number 11, November 1998 , pp. 935-940(6)
Abstract:SETTING: A commercially available polymerase chain reaction (PCR) test (Roche Amplicor™ Mycobacterium avium and M. intracellulare assay–MAC-PCR) designed to detect M. avium complex (MAC) in bronchial washing was evaluated.
DESIGN: A total of 141 specimens from 127 patients with various pulmonary conditions were examined. Results were compared with acid-fast smears, cultures with Ogawa egg medium, as is still commonly used in Japan, and final diagnoses.
RESULTS and CONCLUSIONS: A total of 14 bronchial washing specimens yielded MAC. Six smear- and culture-positive specimens were all MAC-PCR positive. In eight smear-negative and culture-positive specimens, six were MAC-PCR positive. The overall sensitivity versus culture was 85.7% (12/14). However, sensitivity might be over-estimated, as there is a lower recovery rate of MAC with egg-based medium compared with liquid media. In 127 patients, 15 were identified as having pulmonary MAC disease, of whom 13 had positive MAC-PCR in bronchial washing. In the remaining 112 patients, MAC-PCR was negative, which suggests that positive MAC-PCR was not a contaminated result. However, in terms of sensitivity and speed, we were unable to show any additional clinical benefit for using MAC-PCR as opposed to liquid media, in which MAC can frequently be detected in 7 to 14 days.
Document Type: Regular Paper
Affiliations: Department of Infection and Inflammation, Chest Disease Research Institute, Kyoto University, Sakyo-ku, Kyoto, Japan
Publication date: November 1, 1998
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