Usefulness of Mycobacterium tuberculosis genomic mutations in the genes katG and inhA for the prediction of isoniazid resistance
Source: The International Journal of Tuberculosis and Lung Disease, Volume 1, Number 4, August 1997 , pp. 365-369(5)
Abstract:SETTING: Mutations in two genes of Mycobacterium tuberculosis, inhA and katG, are known to correlate with resistance to isoniazid (INH).
OBJECTIVE: To determine which mutation or mutations are the most predictive for INH resistance and the most frequent ones in such isolates. Further, to propose a simple and generally applicable method for their detection.
DESIGN: Codons 94 and 95 in the inhA gene and codons 315 and 463 in the katG gene were characterized in 50 INH-resistant and 12 INH-sensitive isolates from Germany and Sierra Leone.
RESULTS: Mutations in codon 315 of the katG gene were detected in 27 of the INH-resistant and none of the INH-sensitive isolates. All mutations in this codon altered an AciI restriction enzyme site. No mutations were found in the investigated codons of the inhA gene.
CONCLUSION: We propose that most INH resistances can be rapidly predicted by a simple AciI restriction enzyme digest of a polymerase chain reaction (PCR)-amplified katG fragment.
Document Type: Regular Paper
Affiliations: 1: Department of Infectious Diseases and Tropical Medicine, University of Munich, Munich, Germany 2: National Reference Center for Mycobacteria, Borstel, Germany 3: Armauer Hansen Institute, Würzburg, Germany 4: Institute for Laboratory Medicine, Central Hospital Gauting, Gauting, Germany
Publication date: August 1, 1997
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