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Storage of recalcitrant seeds: a case study of the Chinese fan palm, Livistona chinensis

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Various seed storage techniques were applied to the recalcitrant seeds of the Chinese fan palm (Livistona chinensis [Jacq.] R. Br.). Fully hydrated seeds were stored in perlite at 5 moisture levels at 15°C, and partially dried seeds were stored at 6 moisture levels at 4 temperatures. Cryopreservation was attempted with both intact seeds and isolated embryos. It was found that L. chinensis seeds can not survive sub-zero temperature storage for even one week, but short-term storage at above-zero temperature is feasible. Moist storage of fully hydrated seeds did not impair seed viability, but led inevitably to germination and fungal growth after one month. Partially dried seeds began to lose viability after 3 months storage. Intact seeds can not survive cryo-exposure at any moisture content. By contrast, embryos were successfully stored at liquid nitrogen temperature after desiccation to below 20% moisture content, and no significant viability loss was found after 2-year cryostorage. The advantages and disadvantages of these storage methods are evaluated.

Document Type: Research Article


Publication date: April 1, 2009

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  • Seed Science and Technology (SST) is one of the leading international journals featuring original papers and review articles on seed quality and physiology as related to seed production, harvest, processing, sampling, storage, distribution and testing. This widely recognised journal is designed to meet the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality.
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