Germination, storage behaviour and cryopreservation of seeds of Champereia manillana (Opiliaceae) and Schefflera octophylla (Araliaceae)
Authors: Chen, S.Y.; Kuo, S.R.; Chien, C.T.; Baskin, J.M.; Baskin, C.C.
Source: Seed Science and Technology, Volume 35, Number 1, April 2007 , pp. 154-164(11)
Publisher: International Seed Testing Association
Abstract:
Germination and storage behaviour including cryopreservation of Champereia manillana and Schefflera octophylla seeds were studied. Freshly harvested, mature seeds of both species germinated > 90% after three weeks incubation at 30/20°C. Seeds of C. manillana survived desiccation to 5.6% moisture content (MC) (fresh weight basis) during storage at 4°C for 18 months without loss of germinability, whereas the seeds desiccated to 11.3% MC decreased germination percentage from 94.7% to 70% during storage at the same condition. Viability of C. manillana seeds with a MC ≥ 5.6% decreased more rapidly during storage at 15°C than at 4°C. Survival of seeds stored at −20°C was lower than that of seeds stored at either 4 or 15°C. However, maximum viability with a MC of 5.6% was maintained in liquid nitrogen (−196°C) storage after 12 months. These characteristics of C. manillana seeds can be considered as intermediate storage behaviour. Seeds of S. octophylla survived desiccation to 6-7% and −20°C storage for 24 months without loss of viability. Seeds of this species with a MC ≤ 6.2% also survived in liquid nitrogen, whereas viability was reduced to about 50% of that of seeds originally dried and not stored in liquid nitrogen. Considering the sensitivity of seeds of this species towards lower desiccation (<6% MC) at −20°C and decrease of viability during the 24-month storage, the seeds of this species can probably be categorized as having an intermediate storage behaviour.Document Type: Research article
Publication date: 2007-04-01
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- By this author: Chen, S.Y. ; Kuo, S.R. ; Chien, C.T. ; Baskin, J.M. ; Baskin, C.C.

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