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Magnetic Cell Separation by Inkjet Printing for Disease Monitoring

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The standard analysis technique for cell sorting, flow cytometry, requires centralized facilities such as tertiary Medical Centers. In the U.S. alone, more than 65 million people live in medically underserved areas, many of which need access to diagnostic procedures for proper management of their disease. The goal of this research is to develop a cell sorting technique that can be done in low-resource settings at a decreased cost to the medical organization and the patient. By combining inkjet printing technology and magnetic labeling of cells it is possible to obtain accurate cell counts needing only a regular optical microscope. Mouse CD4+ lymphocytes were attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells were attached to a glass slide covering a permanent magnet. The cell counts for this study were obtained by use of regular and inverted optical microscopes and NIS-Elements AR imaging software. Comparisons to flow cytometry will be presented. This novel technique may improve upon existing technologies by reducing costs of training personnel, acquisition and maintenance of instrumentation, and time to conduct analysis
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Document Type: Research Article

Publication date: 2011-01-01

More about this publication?
  • For more than 30 years, IS&T's series of digital printing conferences have been the leading forum for discussion of advances and new directions in 2D and 3D printing technologies. A comprehensive, industry-wide conference that brings together industry and academia, this meeting includes all aspects of the hardware, materials, software, images, and applications associated with digital printing systems?particularly those involved with additive manufacturing and fabrication?including bio-printing, printed electronics, page-wide, drop-on-demand, desktop and continuous ink jet, toner-based systems, and production digital printing, as well as the engineering capability, optimization, and science involved in these fields. In 2016, the conference changed its name formally to Printing for Fabrication to better reflect the content of the meeting and the evolving technology of printing.

    Please note: For purposes of its Digital Library content, IS&T defines Open Access as papers that will be downloadable in their entirety for free in perpetuity. Copyright restrictions on papers vary; see individual paper for details.

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