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Magnetic Cell Separation by Inkjet Printing for Disease Monitoring

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Abstract:

The standard analysis technique for cell sorting, flow cytometry, requires centralized facilities such as tertiary Medical Centers. In the U.S. alone, more than 65 million people live in medically underserved areas, many of which need access to diagnostic procedures for proper management of their disease. The goal of this research is to develop a cell sorting technique that can be done in low-resource settings at a decreased cost to the medical organization and the patient. By combining inkjet printing technology and magnetic labeling of cells it is possible to obtain accurate cell counts needing only a regular optical microscope. Mouse CD4+ lymphocytes were attached to micron sized magnetic beads and printed through a modified, commercial inkjet printer. The labeled cells were attached to a glass slide covering a permanent magnet. The cell counts for this study were obtained by use of regular and inverted optical microscopes and NIS-Elements AR imaging software. Comparisons to flow cytometry will be presented. This novel technique may improve upon existing technologies by reducing costs of training personnel, acquisition and maintenance of instrumentation, and time to conduct analysis

Document Type: Research Article

Publication date: January 1, 2011

More about this publication?
  • For more than 25 years, NIP has been the leading forum for discussion of advances and new directions in non-impact and digital printing technologies. A comprehensive, industry-wide conference, this meeting includes all aspects of the hardware, materials, software, images, and applications associated with digital printing systems, including drop-on-demand ink jet, wide format ink jet, desktop and continuous ink jet, toner-based electrophotographic printers, production digital printing systems, and thermal printing systems, as well as the engineering capability, optimization, and science involved in these fields.

    Since 2005, NIP has been held in conjunction with the Digital Fabrication Conference.

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