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Characterizing spatial distribution of ink-jet printed horseradish peroxidase on paper substrates

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In solid phase bioanalysis, bioagents are incorporated onto solid materials to give them the ability to detect and/or quantify specific target analytes. Previous research has shown that the enzyme horseradish peroxidase (HRP) can be printed on paper without significant inactivation using piezoelectric ink-jet technologies. However, bioanalytical performance of the resulting bioactive papers can be affected not only by the printing conditions and quantity of enzyme deposited, but also by the enzyme spatial distribution and printed enzyme stability. In this study, confocal laser scanning microscopy (CLSM) is used to characterize the inplane and cross-sectional localized enzyme activity of HRP ink-jet printed on fibrous supports with different surface chemistry and composition. Key paper attributes that can affect the analytical performance are identified.
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Document Type: Research Article

Publication date: 2008-01-01

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  • For more than 30 years, IS&T's series of digital printing conferences have been the leading forum for discussion of advances and new directions in 2D and 3D printing technologies. A comprehensive, industry-wide conference that brings together industry and academia, this meeting includes all aspects of the hardware, materials, software, images, and applications associated with digital printing systems?particularly those involved with additive manufacturing and fabrication?including bio-printing, printed electronics, page-wide, drop-on-demand, desktop and continuous ink jet, toner-based systems, and production digital printing, as well as the engineering capability, optimization, and science involved in these fields. In 2016, the conference changed its name formally to Printing for Fabrication to better reflect the content of the meeting and the evolving technology of printing.

    Please note: For purposes of its Digital Library content, IS&T defines Open Access as papers that will be downloadable in their entirety for free in perpetuity. Copyright restrictions on papers vary; see individual paper for details.

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