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Bio-printing of living organized tissues using an inkjet technology

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This study is directed towards developing a novel and versatile method for creating complex tissue structures that use simultaneous ink-jetting of multiple cell types. To demonstrate this concept, 3 different cell types, consisting of human amniotic fluid-derived stem cells (hAFSC), smooth muscle cells (SMC), and bovine aortic endothelial cells (EC), were printed by a thermal inkjet printer to form a heterogeneous cell configuration. Each of the printed cell types maintained viability, proliferation, phenotypic expression, and normal physiological function within the complex structures in-vitro. The bio-printed constructs were able to survive and mature into functional tissues with adequate vascularization in vivo. These findings demonstrate that fabrication of complex hybrid tissue structures that require multiple cell types can be achieved by using the inkjet technology.
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Document Type: Research Article

Publication date: 2006-01-01

More about this publication?
  • For more than 30 years, IS&T's series of digital printing conferences have been the leading forum for discussion of advances and new directions in 2D and 3D printing technologies. A comprehensive, industry-wide conference that brings together industry and academia, this meeting includes all aspects of the hardware, materials, software, images, and applications associated with digital printing systems?particularly those involved with additive manufacturing and fabrication?including bio-printing, printed electronics, page-wide, drop-on-demand, desktop and continuous ink jet, toner-based systems, and production digital printing, as well as the engineering capability, optimization, and science involved in these fields. In 2016, the conference changed its name formally to Printing for Fabrication to better reflect the content of the meeting and the evolving technology of printing.

    Please note: For purposes of its Digital Library content, IS&T defines Open Access as papers that will be downloadable in their entirety for free in perpetuity. Copyright restrictions on papers vary; see individual paper for details.

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